25/02/13How should the size of the camera image be calculated?
1. How to calculate the magnification of the image on the screen
Magnification = Objective lens magnification X C-interface adapter magnification X image diagonal length / sensor diagonal length
2. How large a field of view can the camera see
Image field range = diagonal length of the camera sensor / multiple of the C-interface adapter
3. Resolution calculation of the optical system
1) With microscopes and cameras, the resolution of the current imaging system:
The resolution of the optical system (Optical Resolution) and the image resolution of the camera, the larger median value determines the resolution of the entire imaging system.
- l For the microscope: Optical Resolution =0.61 λ / NA. Where λ is the wavelength (for the fluorescence microscope, it is the emission wavelength of the fluorescence probe). NA is the numerical aperture of the objective lens (which can be found on the objective lens).
- l For the camera: Image Resolution = 2 * pixel size / magnification.
2) With an existing microscope, how to select the camera from the perspective of imaging resolution:
Taking the emitted light with wavelength λ = 509 nm as an example, when using a microscope with different magnification, the optical limit resolution and the corresponding camera sensor image dimensions are matched in the following table:
4. How many times the camera needs to use with a C-interface adapter
Cameras of different sensor sizes need to match C-interface adapters with different cylinder multiples to obtain the best imaging field of vision. For the sensor size and barrel multiple tables, please refer to the following figure:
Theoretical supporting scheme (generally not recommended, may bring the image black edge)
Recommended supporting scheme for use
