Early this year, a paper issued by Peter H. Siegela and Victor Pikovb(a:California Institute of Technology, Pasadena, CA 91125 USA Ø b:Huntington Medical Research Institutes, Pasadena, CA 91105 USA) catching our eyes, TUCSEN cooled camera plays an important role in this research project, here comes extract of this paper:
Keywords: Millimeter Waves, Cells, Neuronal Activity, Non-Invasive
Affecting Neuronal Permeability In Vitro
In addition to H1299 cells, a neuronal cell line B104 (a gift from Peter W. Vanderklish, Scripps Institute) was transfected with fGFP using a plasmid and the expression was stabilized over ten weeks (Fig. 11).
Fig.11. Neuronal cell line transfected with pEGFP-F and expressing fGFP in the membrane.
In order to reduce the acquisition time for the FRET signal, we placed two sensitive photomultiplier tubes (Hamamatsu H5784-20 PMT) in the binocular focus tubes of the microscope for red/green differential amplitude measurement. In front of one of the two PMTs, we placed a red band pass filter (3RD610-650, Omega), and in front of the other a green filter (3RD510-550, Omega). PMT signals were digitized and continuously recorded through a LabView™ program. The CCD camera (TCC-3.3ICE-N, Tucsen Imaging Technology) was used for coincident monitoring of cellular location. PMT measurements on the B104 cells did not fully replicate our earlier findings in the H1299 cells during exposures to similar RF power densities (10mW/cm2 at 60 GHz). Although there was an observed shift from green to red fluorescent signature, indicating the membrane depolarization, no return to baseline, indicating repolarization, was observed. Subsequent discussions with Professors Vergara and DiFranco (UCLA) pointed toward possible enhanced solubility of oxonol in PBS upon millimeter-wave exposure resulting in a permanent increase in the red background signal. A typical PMT measurement, shown in Fig. 12, indicates a sustained FRET process (increase in the red channel). The observed decrease in the red signal seen in the figure was attributed to photobleaching.
Referrence: Proc. of SPIE Vol. 7562 75620H-13
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